Nisha, M. Afrin and Preetha, S. and Selvaraj, J. and Sridevi, G. (2021) Effect of Kabasura Kudineer Extract on Inflammatory Cytokines [Interleukin-6 and Tumour Necrosis Factor-α] in Lung Cancer Cell Line A549. Journal of Pharmaceutical Research International, 33 (47B). pp. 653-660. ISSN 2456-9119
3962-Article Text-5786-1-10-20221006.pdf - Published Version
Download (437kB)
Abstract
Background: Kabasura kudineer is widely known for its anticancer efficiency. Kabasura kudineer is a customary formulation used by siddha practitioners for effectively managing common respiratory illness. Herbal medicines are acknowledged as a great approach to lung cancer therapy. Aim of the study is to know about the anticancer property of Kabasura kudineer extract on inflammatory cytokines IL-6 and TNF-α in lung cancer cell line (A549).
Materials and Methods: Human lung cancer cell line (A549) was purchased from National Centre for Cell Sciences (NCCS), Pune, India. Cell viability test was done by MTT assay. Gene expression analysis was done by Real Time-PCR. The obtained data were analysed statistically by one-way analysis of variance and Duncan’s multiple range test with Graph Pad Prism version 5 to analyse the significance. The significance was considered at p<0.05 level in Duncan’s test.
Results and Discussion: Kabasura kudineer caused a marked increase in cell death in dose dependent manner. AT the end of 48 hours, maximum inhibition was at 400 and 500 μg/ml. Kabasura kudineer extract reduced the expression of IL-6 and TNF-α compared to the control cells.
Conclusion: This study concluded that Kabasura kudineer extract has anticancer activity on lung cancer cell lines (A549).
Item Type: | Article |
---|---|
Subjects: | Librbary Digital > Medical Science |
Depositing User: | Unnamed user with email support@librbarydigit.com |
Date Deposited: | 30 Jan 2023 11:29 |
Last Modified: | 07 May 2024 05:27 |
URI: | http://info.openarchivelibrary.com/id/eprint/18 |