Rahimzadeh, Golnar and Saeedi, Majid and Nokhodchi, Ali and Moosazadeh, Mahmood and Ghasemi, Maryam and Rostamkalaei, Seyyed Sohrab and Mortazavi, Parham and Eghbali, Mohammad and Pourbakhshian, Reza and Rezai, Mohammad Sadegh and Nemati Hevelaee, Ebrahim (2020) Evaluation of in-situ gel-forming eye drop containing bacteriophage against Pseudomonas aeruginosa keratoconjunctivitis in vivo. BioImpacts, 11 (4). pp. 281-287. ISSN 2228-5660
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Abstract
Introduction: Eradication of Pseudomonas aeruginosa has become increasingly difficult due to its remarkable capacity to resist antibiotics. Bacteriophages have been suggested as an alternative treatment for bacterial infections.
Methods: In-situ gel-forming eye drop containing phage against P. aeruginosa keratoconjunctivitis was prepared. The Cystoviridae phage was formulated as in-situ gel-forming formulation which is a solution formulation but turns into gel when it contacts the eye. Therapeutic effectiveness of the in-situ gel forming formulation was evaluated by histological examination on day 12 post-infection.
Results: The viscosity of selected formulation increased when it was instilled into the eye. The histological results showed edema, abscesses, and destruction of the stromal structure of cornea in groups where no in-situ gel-forming formulation was used. In the group where in-situ gel forming formulation was used, re-epithelialization and normal corneal structure were observed.
Conclusion: In-situ gel-forming ophthalmic formulation containing phage can be effective in the treatment of P. aeruginosa keratoconjunctivitis.
Item Type: | Article |
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Subjects: | Librbary Digital > Medical Science |
Depositing User: | Unnamed user with email support@librbarydigit.com |
Date Deposited: | 03 Apr 2023 08:51 |
Last Modified: | 29 Jul 2024 11:24 |
URI: | http://info.openarchivelibrary.com/id/eprint/383 |