BisGMA and TEGDMA Elution from Two Flowable Nanohybrid Resin Composites: An In vitro Study

Aim: The aim of our study was to evaluate the amount of release of BisGMA and TEGDMA from two commercially available enamel replacement composites; Tetric N-Flow™ and G-aenial Universal Flo™ over a period of 24 hours after polymerization with a standard LED Curing Unit.
Methods and Materials: Two flowable nanohybrid composite materials; Tetric N-Flow™ (Ivoclar Vivadent AG, Liechtenstein) and G-aenial Universal Flo™ (GC, India) were investigated and grouped into two groups. Ten samples from each group were prepared by inserting the material into a standardized Teflon mould of size 2x2x2 mm. Each sample was cured with a LED curing unit for 20 seconds and was stored in 2 ml of Ethanol at room temperature. After 24 hours, the samples were removed from the storage medium (ethanol) and prepared for measurements. A reverse phase HPLC unit was used to detect the release of BisGMA and TEGDMA monomers from the prepared samples. The acquired measurements were obtained after testing them in a High Liquid Performance Chromatography Unit. The data obtained was statistically analyzed and the results revealed significant amount of release of TEGDMA as well as BisGMA.
Results: G-aenial Universal Flo™ showed significant release of both TEGDMA as well as BisGMA as compared to Tetric N- Flow™. The increase was by 0.5 units.
Conclusion: Significant amount of release of TEGDMA as well as BisGMA was seen in both the composite materials after HPLC Unit analysis. This can help in the evaluation of cytoxicity to the soft tissues in the oral environment.